Located along the Canadian border in central Alaska, the preserve protects 115 miles of the 1,800-mile Yukon River and the entire Charley River basin. Numerous rustic cabins and historic sites are reminders of the importance of the Yukon River during the 1898 gold rush. Paleontological and archeological sites here add much to our knowledge of the environment thousands of years ago. Peregrine falcons nest in the high bluffs overlooking the river, while the rolling hills that make up the preserve are home to an abundant array of wildlife. The Charley, a 100-mile long wild river, is considered by many to be the most spectacular river in Alaska.

The Museum of Alaska Transportation and Industry (MATI) started in 1967 as the Air Progress Museum. It was a small collection of Alaskan transportation artifacts gathered for the centennial celebration of the United States purchasing Alaska from Russia in 1867. The Museum of Alaska Transportation and Industry today has over 20 acres, encompassing a large gallery, train yard, rows and rows of outdoor artifacts, and an exhibit hall.

This park celebrates the Klondike Gold Rush of 1897-98 through 15 restored buildings within the Skagway Historic District. The park also administers the Chilkoot Trail and a small portion of the White Pass Trail. Included in the park is a portion of the Dyea Townsite at the foot of the Chilkoot Trail.